LOCUS       pLV-[hsa-miR-921]        9190 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7111..7971
                     /label=Amp(R)
     promoter        8172..8712
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5716..5950
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6525..6980)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6022..6152
                     /label=SV40 pA
     misc_feature    6153..6366
                     /label=SV40 ori
     misc_feature    5037..5633
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3655
                     /label=intron
     CDS             3702..5027
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3445
                     /label=hsa-mir-921 precursor
BASE COUNT     2178 a      2392 c      2498 g      2122 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca atggccagaa 
     3121 gaatgcaagt ggtagatcaa cagaatcatt cttaacttac agggtgttag gatggtgcgg 
     3181 gaaatcatct agctcatttg cttcttttac agatggggac gctgaagccc agaaaagggc 
     3241 agtgccacaa ctagtgaggg acagaaccag gattcagact caggtccatg ggcctggatc 
     3301 actggctaat tctgtgggtc agggctggag gcacccagca cacctagtct tcaaagcagc 
     3361 agtcagtggt cacctccccc tgttttttca tcactgcctg cccctgcccc ccagccaggt 
     3421 ttgctgaggg tccaggtctt caggcgctag ctcgagcttt tggagtacgt cgtctttagg 
     3481 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt 
     3541 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc 
     3601 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc 
     3661 gtgaaaacta cccctctaga gtcgagctac cggtcgccac catggtgagc aagggcgagg 
     3721 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 
     3781 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 
     3841 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 
     3901 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 
     3961 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 
     4021 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg 
     4081 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg 
     4141 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 
     4201 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 
     4261 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 
     4321 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 
     4381 ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg accgagtaca 
     4441 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 
     4501 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 
     4561 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 
     4621 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 
     4681 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 
     4741 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 
     4801 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 
     4861 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 
     4921 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc 
     4981 cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg tgcctgagcg gccgcgtcga 
     5041 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 
     5101 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 
     5161 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 
     5221 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 
     5281 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 
     5341 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
     5401 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 
     5461 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 
     5521 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 
     5581 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg 
     5641 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 
     5701 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt 
     5761 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 
     5821 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 
     5881 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 
     5941 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 
     6001 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 
     6061 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     6121 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 
     6181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     6241 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     6301 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc 
     6361 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 
     6421 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 
     6481 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 
     6541 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     6601 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     6661 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     6721 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     6781 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     6841 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 
     6901 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     6961 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     7021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     7081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     7141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     7201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     7261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     7321 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     7381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     7441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     7501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     7561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     7621 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     7681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     7741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     7801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 
     7861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     7921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     7981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     8041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     8101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     8161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     8221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     8281 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     8341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     8401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     8461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     8521 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     8581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     8641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     8701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     8761 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 
     8821 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 
     8881 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 
     8941 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 
     9001 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 
     9061 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 
     9121 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag 
     9181 ctggagctgc 
//
